Pyrosequencing Technology for Methylation Analysis
EpigenDx is the world-wide leading Pyrosequencing service provider in the biotechnology industry. Our lab has nearly fifteen years of expertise in Pyrosequencing technology and assay design. We offer quality services, excellent customer support, and rapid turnaround time. Our design team is skilled in working with even the most difficult regions for epigenomic applications.

Over 7000+ Validated Assays Offered
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A Real-Time and Quantitative Solution
Pyrosequencing is EpigenDx’s choice platform for loci-specific DNA methylation analysis since it is fast, cost-effective, and can accurately provide real-time quantitative percent methylation reads. This makes it ideal for applications involving allele quantification. A single Pyrosequencing run for DNA methylation is equivalent to sequencing 20 clones with traditional Sanger Bisulfite Sequencing.
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Loci-Specific DNA Methylation
Measure the percent methylation levels of all individual CpG sites in a target region (PCR amplicon).
Global DNA Methylation
Assess the average amount of DNA methylation globally in a sample.
Allele-Specific Quantitation
Quantify the amounts of two alleles at a basepair resolution.
SNP Genotyping
SNP and mutation genotyping via Pyrosequencing technology.
Custom Assay Design
Send us a custom target region with your gene of interest. We will design and validate the assay.
The Services Include:
Items | Service Products | Description | Order |
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8001 | DNA Methylation Analysis – Standard | Quantitative Methylation Analysis on submitted bisulfite-converted PCR products
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Inquiry / Order |
8002 | DNA Methylation Analysis – Gold | Quantitative Methylation Analysis on submitted bisulfite modified DNA
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Inquiry / Order |
8003 | DNA Methylation Analysis – Premium | Quantitative Methylation Analysis on submitted genomic DNA
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Inquiry / Order |
8005 | Bisulfite modification | Bisulfite modification on submitted genomic DNA
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Inquiry / Order |
8006 | Assay Design – Standard | Pyrosequencing primer design for DNA methylation, SNP genotyping, and allele specific expression analysis:
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Inquiry / Order |
8008 | Assay Design – Premium | Design and optimize PCR and Pyrosequencing primers for DNA methylation analysis, SNP genotyping, and allele specific expression analysis:
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Inquiry / Order |