Targeted Next-Gen Bisulfite Sequencing for DNA Methylation Analysis
EpigenDx specializes in targeted next-generation sequencing (NGS) services for affordable, fast, and precise sequencing of custom target regions. Our targeted bisulfite sequencing services can provide methylation data for dozens of genes and entire promoter regions in a single run.
Choose from pre-validated panels or utilize our design services to develop a custom library covering your targets of interest. All of our off-the-shelf panels are validated with Pyrosequencing technology.
We use Ion Torrent™ technology for targeted gene sequencing and methylation analysis.
Advantages
Targeted Next-Gen Bisulfite Sequencing is more economical and has a faster turnaround time than whole-genome methylation analysis (WGBS or RRBS). It allows you to analyze only the regions and genes you are interested in, and produces higher quality data due to significantly greater coverage of each CpG site.
EpigenDx offers a complete solution for targeted NGS. We perform the DNA extraction, bisulfite modification, library preparation, high throughput sequencing, data analysis, and assay validation with Pyrosequencing. We are the experts in DNA methylation analysis with over 15 years of experience in bisulfite sequencing.
Applications
DNA Methylation Analysis
- Measure the percent methylation of multiple target regions (PCR amplicons) and multiple samples in a single run.
- Ability to perform DNA methylation analysis covering many areas of one gene or a diverse set of regions across multiple genes
Illumina’s MethylationEPIC BeadChip Assay Validation
- Validate methylation data obtained on Illumina’s MethylationEPIC or 450K microchip array
- Use targeted Next-Gen bisulfite sequencing (tNGBS) to select and validate 25-500 loci at a time.
Pre-Designed Methylation Panels
Use our pre-validated off-the-shelf methylation panels to assess the methylation levels of genes related to cancer, pluripotency, and more.
Custom Panel Design
Send us custom target regions with your genes of interest. We will design and validate a custom panel of assays.
Our Services:
| Items | Service Products | Description | Order |
|---|---|---|---|
| 8200 | Custom NGS Assay Design | Assay design based on customer-submitted target region(s).
|
Inquiry / Order |
| 8500 | Targeted Next-Gen Sequencing – Standard | Custom target gene sequencing using Ion Torrent instruments:
|
Inquiry / Order |
| 8501 | Targeted Next-Gen Sequencing – Premium | Custom target gene sequencing using Ion Torrent instruments:
|
Inquiry / Order |
| 8100a | NGS Data Analysis | Preliminary data analysis of NGS generated sequencing data. | Inquiry / Order |