Immunology Methylation Panel
Analysis of the percentage methylation levels of multiple genomic loci showing the relationship between immunology and DNA methylation
The Immunology Methylation Panel measures methylation levels of CpG sites at multiple regions in the genome important for the function of the immune system. We achieve this by bisulfite modification and multiplex PCR followed by Next Generation Sequencing.
The assays in this panel are pre-validated to ensure no preferential amplification for either methylated or unmethylated DNA, providing the quantitation of DNA methylation at base pair resolution.
Step 1: Select Your Targets
Choose from our carefully designed panel of 16 genes, and choose some of your own targets. We will design and validate any additional assays you choose to add to the panel.
For Treg-related genes,
see our FOXP3 Methylation Panel
|Information: Immunology Methylation Panel|
|Species:||Human (Homo sapiens)|
|Stats:||95 CpG sites over 16 genes using 20 amplicons|
|Full Gene List:||TNFRSF25, IL2, IL4, IL7R, CD247, CD40, IL1B, TNFRS10C, IL8, CD44, CXCR3, TNF, IL13, MMP1, MMP3, MMP13|
|Panel Summary:||Download our full list of NGS methylation assays offered in the Immunology Methylation Panel.|
Interleukins are a large group of small signaling peptides, or cytokines, involved in the function of white blood cells.
This panel covers several major families of interleukins and their corresponding receptors, including:
- IL1B – promotes inflammation
- IL2 – regulates and promotes the differentiation of T cells into regulatory, effector and memory T cells
- IL4 and IL13 – promotes the proliferation of activated helper T cells and B cells
- IL7R – promotes the early maturation of lymphoid cells and genetic recombination
- IL8 – recruits white blood cells to the site of inflammation
Proinflammatory cytokines are small peptides that promote inflammation in the innate immune response.
This panel covers two major proinflammatory cytokines:
- IL1B – produced primarily by macrophages, monocytes and endothelial cells to upregulate the inflammatory response
- TNF – (tumor necrosis factor) formerly known as TNF alpha, mediates the inflammatory response
Cell surface proteins often help the immune system to distinguish “self” from “non-self” in addition to acting as receptors.
This panel covers some major surface proteins:
- CD247 – also known as the T-cell receptor T3 zeta chain, is a component of the T-cell receptor-CD3 complex that recognizes fragments of antigen bound to the major histocompatibility complex (MHC) on antigen-presenting cells (APC)
- CD40 – is a surface protein on antigen-presenting cells that binds to CD154 (CD40L) on helper T cells causing their activation
- CD44 – involved in cell-cell adhesion and is used, along with CD25, to identity early differentiated T cells in the thymus
Chemokines are cytokines that induce chemotaxis, or movement of cells in response to a chemical stimulus.
This panel covers a few chemokines:
- IL8 – causes macrophage migration to the site of inflammation
- CXCR3 – a chemokine receptor that regulates leukocyte trafficking, especially of Th1 cells
The tumor necrosis factor superfamily of proteins are cytokines initially observed to cause apoptosis.
This panel’s tumor necrosis factors and receptors:
- TNFRSF25 – also known as death receptor 3, mediates apoptosis
- TNFRS10C – antagonistic receptor that protects cells from apoptosis
- TNF – multifunctional proinflammatory cytokine
Matrixins are enzymes that break down extracellular matrix proteins such as collagen.
This panel covers a few matrixins:
- MMP1 – matrix metalloproteinase-1 breaks down interstitial collegens, type I, II, III
- MMP3 – matrix metalloproteinase-3 also known as stromelysin-1 degrades various extracellular matrix proteins and activates other MMPs
- MMP13 – matrix metalloproteinase-13 also known as collagenase 3 participates in collagen degradation
There are many genes related to regulatory T-cell (Treg) differentiation and function. See EpigenDx’s FOXP3 Methylation Panel for more details.
Can’t find your gene of interest? Contact us! EpigenDx has over seven thousand individually pre-validated assays, most of which have not yet been posted on this website. Please let us know if you cannot find what you are looking for.
Step 2: Choose an Ideal Plan for You
Choose the services that are appropriate and ideal for your needs. All parts of our methylation services are customizable and modular. We offer services in:
|8200||Custom NGS Assay Design||Assay design based on customer-submitted target region(s).
||Inquiry / Order|
|8500||Targeted Next-Gen Sequencing – Standard||Custom target gene sequencing using Ion Torrent instruments:
||Inquiry / Order|
|8501||Targeted Next-Gen Sequencing – Premium||Custom target gene sequencing using Ion Torrent instruments:
||Inquiry / Order|
|8100a||NGS Data Analysis||Preliminary data analysis of NGS generated sequencing data.||Inquiry / Order|
Step 3: Results
EpigenDx analyzes the raw data and delivers:
- The percentage methylation values of each CpG site in a Microsoft Excel spreadsheet.
- Sequencing data in a FASTQ file.
Interpretation of Data
Methylation data can then be applied to research. For example, increased DNA methylation at specific CpG sites in the promoter region of genes is a hallmark of many cancers.
Speak with experts at EpigenDx for high quality technical support and consultation through all phases of the project, from assay design to data delivery.