FOXP3 Methylation Panel
Regulatory T-cells (Tregs) prevent autoimmune disease by inhibiting self-reactive T-cells. Tregs are known to exhibit a unique epigenome and express the transcription factor FOXP3, which is a biomarker that distinguishes Tregs from other non-regulatory T-cells. EpigenDx’s FOXP3 panel is a set of validated DNA methylation assays targeting genes that exhibit unique methylation profiles in Tregs.
The FOXP3 Methylation Assays cover a total of 32 human gene regions, 15 mouse gene regions, 1 rhesus monkey gene region. We can analyze the entire panel using targeted Next-Gen bisulfite sequencing. Or, we can analyze select PCR regions using Pyrosequencing. The panel is customizable to exclude or include any other target regions of your interest.
Full List of Human FOXP3 Methylation Panel Assays
FOXP3 Panel Available as Kits or Services
Information: Human FOXP3 Methylation Panel | |
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Platform: | Targeted Next-Gen Bisulfite Sequencing Pyrosequencing for analyzing a selected gene and a target region within this panel |
Species: | Human (Homo sapiens) |
Stats: | Greater than 120 CpG sites over 18 genes and 25 amplicons |
Full Gene List: | CD274, CD3D_CD3G, CDKN1C, CTLA4, ETS1, FOXP3, IKZF2, IKZF4, IL2, IL2RA, IL2RB, IL4, PDCD1, PDL2, RUNX1, S100A6, TNFa, TNFRSF18, TNFRSF25, TOLLIP |
Information: Mouse Foxp3 Methylation Panel | |
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Platform: | Targeted Next-Gen Bisulfite Sequencing Pyrosequencing for analyzing a selected gene and a target region within this panel |
Species: | Mouse (Mus musculus) |
Stats: | Greater than 100 CpG sites over 7 genes and 12 amplicons |
Full Gene List: | Foxp3, Ctla4, Helios (Ikzf2), Eos (Ikzf4), Il2ra (Cd25), Tnfrsf18 (Gitr), Cd274, and Irf4 |
EpigenDx FOXP3 Panel Methylation Analysis Service
- Send your samples to EpigenDx’s service laboratory.
- Ability to accept all types of DNA (Serum, FFPE, etc.).
- Versatility with Pyrosequencing and targeted Next-Gen Bisulfite Sequencing
- EpigenDx provides a complete solution: DNA extraction, bisulfite modification, library preparation, sequencing, and data analysis.
- Full result reports with an explanation of the data.
FOXP3 Panel Assay Kits
- Primers compatible with IonTorrent™, all major NGS and Pyrosequencing technologies.
- Primers good for 300 reactions.
- Access to our knowledgeable and experienced customer service.
Human FOXP3 Methylation Panel: NGS Data Sheet
Detailed Information About the NGS Human FOXP3 Panel
- EpigenDx uses targeted Next-Generation Bisulfite Sequencing to analyze the entire panel in one sequencing run.
- The panel is customizable to include or exclude custom regions.
- Please download the data sheet for a comprehensive list of assays included in the methylation panel.